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产品目录

Bpu14I内切酶图片
产品货号:
GS2078
中文名称:
Bpu14I内切酶
英文名称:
Bpu14I
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesTT↑CGAA
来源Bacillus pumilus 14
Assayed onLambda DNA
Optimal bufferG(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃.
LigationsAfter 10-fold overdigestion with enzyme about 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsAsuII,BsiCI,Bsp119I,BspT104I,BstBI,Csp45I,NspV,SfuI

相关搜索:Bpu14I内切酶Bpu14I限制性内切酶
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